The tournament, for clubs in npower League One and Two, will kick off in September and end with a Final at Wembley Stadium in April.
Brentford have reached the Final of the competition on three occasions - in 1985, 2001 and 2011 - but have yet to win it.
Last year Uwe Rösler's side were beaten in the Southern Section Semi-Final by Barnet after a penalty shoot-out.
The Bees will be in the Southern Section again this year - full details of the sections and the eight first round byes will be revealed in due course.
The draw for each round up to the Area Semi-Finals will be live on Sky Sports' Soccer AM programme.
The dates for the round, and draws, are as follows:
First Round - w/c September 3 2012 (Draw date Saturday August 18 2012)
Second Round - w/c October 8 2012 (Draw date Saturday September 8 2012)
North/South Quarter Final - w/c December 3 2012 (Draw date Saturday October 13 2012)
North/South Semi Final - w/c January 7 2013 (Draw date Saturday December 82012)
North/South Final First Leg - w/c February 4 2013
North/South Final Second Leg - w/c February 18 2013
FINAL - Sunday April 7 2013