Anna Gelderblom scored twice in vain as Brentford WFC Development were beaten 4-2 by Alexandra Park on Sunday.
With the first-team game against Hampton and Richmond Ladies called off due to ground maintenance work at The Beveree, attention switched to the development team on Sunday.
Justin Ali's side made the trip to The Racecourse Ground having won two of their last three in the Greater London Women's Football League Division Three North. However, the hosts had made an impressive unbeaten start to the season and proved too strong on the day.
Jennifer Moore scored a hat-trick with Sara Lugo adding the other as the North London side made it five league wins from six this campaign despite midfielder Gelderblom's brace. Defeat leaves The Bees in eighth ahead of a cup trip to Balham Panthers next weekend.
Craig Brown's first-team are again without a fixture next weekend but return to action at Lampton School on Sunday 22 October when they host top of the table New London Lionesses.