A goalless draw seemed to be going to a penalty shootout but a late finish from Shaun Lucien meant the young Bees would fall to defeat, despite creating a number of chances throughout.
Neil MacFarlane named first teamer Ellery Balcombe in his starting line-up amongst a youthful XI with Julien Carre coming into the midfield following Arthur Read’s loan to Stevenage.
Brentford B began dominating much of the early possession but were unable to make it count in terms of creating clear chances. Carre was first to try his luck from distance as he shaped onto his right foot, but the shot went just over the Hendon goal.
An Aaron Pressley header at the far post following a corner was diverted goalwards as he stooped to connect, but the Hendon defenders blocked well on the line.
The opponents carried a threat on the counterattack throughout and created a good opportunity when Shaun Lucien darted down the left before firing powerfully but for a good block from Paris Maghoma.
Half chances followed for the likes of Pressley and Kane O’Connor headed just over following another corner, but Brentford B struggled to create many clear-cut opportunities as Hendon worked hard to keep them out.
A Maghoma shot from distance would be well saved before O’Connor was called upon to make a vital defensive header following a vicious Hendon strike at goal as the sides went into the break level.
The second period followed a similar formula with the young Bees often seeing more of the ball and trying to break their opponents down whilst Hendon offered a rare threat on the counter.
Maghoma would have another attempt, this time from a free-kick following a foul on Fredrik Hammar, but the youngster’s dipping effort was the wrong side of the crossbar. Joe Adams, who threatened down the left, would have an opportunity on 66 minutes when he turned well before seeing his effort edge just wide of goal.
The Brentford Trialists were pushing to make an impact on the game, and one of them almost did when he received the ball on the edge of the area before turning well and seeing his effort deflect, and with the keeper rooted to the spot, the ball nestled just wide of the bottom left-hand corner.
Lucien would have one notable effort for Hendon when he used his speed to break away, but his finish lacked conviction as he shot low but wide of goal as Balcombe was untested as the match headed to extra-time.
It took The Bees just three minutes to register a really good opportunity and it came when one of the Trialists fired at goal and the Hendon keeper parried into the path of Pressley whose follow up effort was saved again by the shot stopper.
Brentford would continue to push for that all-important winner, but the game swung in the second period of extra-time when Kane O’Connor fouled in the box just nine minutes from time and the referee had no hesitation in pointing to the spot.
Lucien stepped up to convert calmly and send Balcombe the wrong way as The Bees rushed to get the ball back to the centre spot. Hendon did well to frustrate in the final moments but a golden chance fell when a very late corner was swung in and Pressley, Balcombe and Tristan Crama all threw themselves at the ball but it nestled wide as Hendon held out.
Hendon FC: North, Simeon, Perry, Forbes, Brewer, Osmodi, Weign, Sambou, James, Corcoran, Gebrai
Subs: Howes, Allinson, Clarke
Brentford B: Balcombe, Stevens, Gordon, O'Connor (Crama, 108) Koutsimouka, Adams, Hammar (Trialist, 75), Pressley, Carre (Trialist, 90), Trialist, Maghoma
Subs: Andersson, Hockenhull