Brentford Football Club has agreed with Leyton Orient an allocation of 2,681 tickets for the potentially vital Sky Bet League One match in East London next month.
The Bees will travel to The Matchroom Stadium for the top-of-the-table clash on Saturday March 15.
The game will kick off at 12.15pm and will be broadcast live on Sky Sports.
After negotiations with Leyton Orient, Brentford has taken the biggest allocation available for the London derby.
The teams are currently first and third in League One and the match could play a role in determining if either team wins promotion at the end of the season.
Mark Devlin, Brentford FC Chief Executive, said: “We knew from very early in the season that this would be a vital game and demand for tickets would be large.
“As the game approaches and supporters talk to the Club about it, this view is reinforced.
“Even with the game on TV we believe Brentford supporters will want to attend and we have asked Leyton Orient for as many tickets as possible.
“They need to move supporters in order to give us this larger allocation and we have had to commit to selling more than 2,100 tickets or paying for them even if we don’t.
“I believe the demand will exceed this number and I would like to think we could sell this game out.
“We hope we can get tickets on sale by the end of the week and more details will follow in due course.
“Season Ticket Holders and Club Members will get priority on purchasing tickets for this match, as with all the big matches we face between now and May.”
Full details concerning ticket prices and sale dates will be announced on www.brentfordfc.co.uk as soon as they are confirmed.